FIGURE 2.

Cyclic stretch exerts direct inhibitory effects on ATP triggering in LPS-primed macrophages. J774.1 cells were primed with 100 ng/ml of E. coli LPS for 4 h and exposed to CS of 20% elongation at a frequency of 10 cycles/min just before the addition of 1 mM ATP for 2 h. The amount of IL-1β in the supernatant was analyzed by ELISA. Representative data of three separate experiments are shown as the means ± SD of triplicate assays. Significance is indicated (^∗P < 0.05 significantly different from the positive control).