Table 2.

Real-Time PCR Program

Step	Temperature	Time	Number of cycles
UNG (uracil-N-glycosylase) pre-treatment	50°C	2 min	1
Initial Denaturation	95°C	10 min	1
Denaturation	94°C	15 sec	45
Annealing	60°C	30 sec
Extension	72°C	30 sec