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Amplification- Refractory Mutation System –PCR Primers for VEGF -2578C>A Gene Polymorphism
| Direction | Primer Sequence | AT | Product size | |
|---|---|---|---|---|
| FO- VEGF | 5-CCTTTTCCTCATAAGGGCCTTAG-3 | 58oC | 353bp | |
| RO- VEGF | 5-AGGAAGCAGCTTGGAAAAATTC-3 | |||
| FI –VEGF (Fwt) | A allele | 5-TAGGCCAGACCCTGGCAA-3 | 149bp | |
| RI- VEGF (Rmt) | C allele | 5-GTCTGATTATCCACCCAGATCG-3 | 243bp |
Fo-outer forward primer, Ro-Reverse outer primer; AT-annealing temperature; FI-Inner forward primer, RI-Inner Reverse primer
