Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Preparation of PCR Cocktail for VEGF -2578C>A Polymorphism
| 1x | |
|---|---|
| PCR master mix | 10ul |
| Forward primer FO | 0.30 ul |
| Reverse primer RO | 0.30 ul |
| Forward primer FI A | 0.20 ul |
| Reverse primer RI C | 0.20 ul |
| Nuclease free water | 12ul |
| Total volume | 23ul |
| DNA (50ng) | 2ul |
