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. 2018;19(5):1277–1280. doi: 10.22034/APJCP.2018.19.5.1277

Table 2.

Primer Sequence and Characteristics Used in the Quantification of the Target Genes

Gene Forward primer Annealing temp (°C)
Caspase 3-Forward 5'-ATGGTTTGAGCCTGAGCAGA-3' 59.5
Caspase 3-Reverse 5'-GGCAGCATCATCCACACATAC-3' 58.5
Caspase 8- Forward 5'-ACCTTGTGTCTGAGCTGGTCT-3' 59.5
Caspase 8- Reverse 5'-GCCCACTGGTATTCCTCAGGC-3' 59
Caspase 9- Forward 5'-GCAGGCTCTGGATCTCGGC-3' 60.5
Caspase 9- Reverse 5'-GCTGCTTGCCTGTTAGTTCGC-3' 59.5
GAPDH- Forward 5'-CAAGATCATCAGCAATGCCTCC-3' 59
GAPDH- Reverse 5'-GCCATCACGCCACAGTTTCC-3' 59