Table 2.
Primer Sequence and Characteristics Used in the Quantification of the Target Genes
Gene | Forward primer | Annealing temp (°C) |
---|---|---|
Caspase 3-Forward | 5'-ATGGTTTGAGCCTGAGCAGA-3' | 59.5 |
Caspase 3-Reverse | 5'-GGCAGCATCATCCACACATAC-3' | 58.5 |
Caspase 8- Forward | 5'-ACCTTGTGTCTGAGCTGGTCT-3' | 59.5 |
Caspase 8- Reverse | 5'-GCCCACTGGTATTCCTCAGGC-3' | 59 |
Caspase 9- Forward | 5'-GCAGGCTCTGGATCTCGGC-3' | 60.5 |
Caspase 9- Reverse | 5'-GCTGCTTGCCTGTTAGTTCGC-3' | 59.5 |
GAPDH- Forward | 5'-CAAGATCATCAGCAATGCCTCC-3' | 59 |
GAPDH- Reverse | 5'-GCCATCACGCCACAGTTTCC-3' | 59 |