Fig. 1.

Comparison of natriuretic and kaliuretic response to hydrochlorothiazide (HCTZ) and furosemide administered via intraperitoneal (ip) vs. subcutaneous (sq) route. A and B: HCTZ or furosemide was administered to wild-type mice through ip injection. Vehicle contains 0.5 ml normal saline and 12.5 μl dimethyl sulfoxide (DSMO; solvent for diuretics) to mice (~25 g). The hourly rate of Na⁺ and K⁺ excretion during 24-h before the injection (Pre-), 0–4 h, and 4–24 h after the injection is shown; n = 4 each. C: the rate of urinary Na⁺ excretion in mice administered with vehicle alone (n = 4) or HCTZ (n = 4) through subcutaneous route. The total amounts of Na⁺ excretion in the urine during each collection period and the daily sums are shown. D: the rate of Na⁺ and K⁺ excretion during each collection period from mice injected subcutaneously with HCTZ; n = 8. Mice were pair-fed with a normal-Na⁺ diet from −2 to +1 days. Diuretics were administered at time 0. *P < 0.05 vs. “Pre” period.