Fig. 2.

Blunting of HCTZ-induced Na⁺ excretion in Ncc-knockout mice. A: primer position for genotyping for the targeted Ncc (Na⁺- Cl⁻ cotransporter) gene. A Neo cassette was inserted into exon 12 of Ncc to disrupt gene function. B: genotyping of WT vs. Ncc-KO mice. C: Western blot analysis of NCC protein in kidney extracts from WT and Ncc-KO mice. D: HCTZ-induced Na⁺ excretion during the acute phase (HCTZ, from 0 to 4 h) after the drug administration was detected in WT (n = 5) mice, but not in the Ncc-KO (n = 5) mice. In Ncc-KO mice, the level of Na⁺ excretion after HCTZ was equivalent to vehicle-treated WT mice (n = 6) (indicated by the horizontal line).