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NaCl loading upregulates the abundance of phospho-NCC in WT as well as Wnk4-KO mice. A: Western blot analysis of p-NCC (T53) from kidney extracts of WT and Wnk4-KO mice under control or NaCl loading. B: semiquantitative analysis of the p-NCC (T53) signal intensity normalized to β-actin signal intensity in each lane. *P < 0.05, NaCl vs. control for respective strain. #P < 0.05, Wnk4-KO vs. WT.
