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Effect of inhibition of TLR4 signaling on monocyte chemoattractant protein-1(MCP-1) mRNA expression in NRK-52E cells exposed to hemin. MCP-1 gene expression was assessed by quantitative real-time RT-PCR after 8 h of exposure to control media or media containing 5 μM hemin, with or without inhibition of TLR4 signaling with TAK-242; n = 4 in each group. *P < 0.05 vs. corresponding vehicle-treated group.
