Fig. 4.

Effect of inhibition of TLR4 signaling on NF-κB activation in NRK-52E cells exposed to hemin. EMSA for the expression of NF-κB was performed using nuclear extracts from NRK-52E cells exposed to control media or media containing 5 μM hemin for 3 h, with or without inhibition of TLR4 signaling with TAK-242. A negative control consisting of a binding reaction in the absence of nuclear extract is displayed in lane 1. Supershifting (lane 10) of the nuclear extract from lane 6 was conducted using a p65 antibody; ns, nonspecific bands.