Fig. 6.

Effect of inhibition of TLR4 signaling on the activation of Nrf2 in NRK-52E cells exposed to hemin. A: representative Western analysis of the nuclear expression of Nrf2 after 3 h of exposure to control media or media containing 5 μM hemin, with or without inhibition of TLR4 signaling with TAK-242. Equivalency of protein loading was assessed by immunoblotting for HDAC1. B: densitometric assessment of the Western analysis of Nrf2 expression; n = 4 in each of the control groups and n = 6 in each of the hemin-treated groups. *P < 0.05 vs. corresponding vehicle-treated group.