Fig. 5.

HG treatment increased the level of ubiquitinated Orai1 protein in rat MCs. A: representative Western blot showing abundance of ubiquitinated Orai1 protein in rat MCs with and without HG treatment. Confluent rat MCs were cultured in 0.5% FBS medium containing 5.6 mM d-glucose and 20 mM α-mannitol (−HG) or 25 mM d-glucose (+HG) for 8 h. PC, positive control, provided by the manufacturer; Ub-Orai1, ubiquitinated Orai1; TB, α-tubulin, the loading control. B: summary densitometric data from experiments presented in A. *P < 0.05, +HG vs. –HG. n Indicates the number of independent experiments.