Table 1.
C20 qPCR assay specificity and sensitivity.
| a. Specificity testing | |||
|---|---|---|---|
| Species | DNA (ng) | Mean Ct Value | SD |
| A. spirocauda (P-Pr-13-104) | 1 | 16.75 | 0.01 |
| A. viteae | 1 | nda | nd |
| D. immitis | 1 | nd | nd |
| H. delphini (P-Pr-12-050) | 1 | nd | nd |
| A. simplex (DO 8135) | 1 | nd | nd |
| B. pahangi | 1 | nd | nd |
| D. odendhali (P-Pr-13-007) | 1 | nd | nd |
| O. circumlitus (P-Pr-13-024) | 1 | nd | nd |
| C. boopis (P-Pr-11-018) | 1 | nd | nd |
| No template control (NTC) |
– |
nd |
nd |
| b. Sensitivity testing | |||
| Species |
DNA (ng) |
Mean Ct Value |
SD |
| A. spirocauda (P-Pr-13-104) | 1 | 15.98 | 0.34 |
| A. spirocauda (P-Pr-13-104) | 0.1 | 23.92 | 0.07 |
| A. spirocauda (P-Pr-13-104) | 0.01 | 28.55 | 0.25 |
| A. spirocauda (P-Pr-13-104) | 0.001 | 35.02 | 1.37 |
| A. spirocauda (P-Pr-13-104) | 0.0001 | 38.58 | 0.02 |
| A. spirocauda (P-Pr-13-104) | 0.00001 | nd | nd |
| A. spirocauda (P-Pr-13-104) | 0.000001 | nd | nd |
| No Template Control (NTC) | – | nd | nd |
a
nd = not detected.