Table 4.
Screening results for mock infected blood samples.
| Sample | Total input DNA (ng) | Mean Ct value | SD |
|---|---|---|---|
| A: Spiked Blood 1 (Whole blood + 1X A. spirocauda)* | 1 | 26.39 | 0.48 |
| B: Spiked Blood 2 (Whole blood + 10X dilution A. spirocauda)* | 1 | 30.43 | 1.26 |
| C: Spiked Blood 3 (Whole blood + 100 X dilution A. spirocauda)* | 1 | nda | nd |
| + ctrl (P-Pr-13-104, A. spirocauda) | 1 | 15.98 | 0.34 |
| + ctrl 10X dilution | 0.1 | 23.92 | 0.07 |
| + ctrl 100X dilution | 0.01 | 28.55 | 0.25 |
| + ctrl 1000X dilution | 0.001 | 35.02 | 1.37 |
| + ctrl 10000X dilution | 0.0001 | 38.57 | 0.02 |
| + ctrl 100000X dilution | 0.00001 | nd | nd |
*Sample A, B, and C were generated by adding A. spirocauda DNA to whole seal blood to a final concentration of 0.05 ng/uL, 0.005 ng/uL, and 0.0005 ng/uL respectively. Total DNA from whole blood containing parasite DNA was then extracted as described in the methods.
a
nd = not detected.