Figure 5.
Live-cell imaging of IpLITR 1.1b-mediated target interactions. Rat basophilic leukemia-2H3 cells (3 × 105) stably co-expressing IpLITR 1.1b and LifeAct-GFP were incubated at 37°C with 9 × 105 αHA monoclonal antibody-coated 4.5 µm microspheres. Immediately after the addition of target beads, images were collected at 10 s intervals for ~8 min using a Zeiss LSM 710 laser scanning confocal microscope (objective 60×, 1.3 oil plan-Apochromat; Munich, Germany). Both the brightfield-LifeAct-GFP merged views (top panels) and the LifeAct-GFP views alone (bottom panels) are shown for two representative (A,B) IpLITR 1.1b-mediated target interactions with the location of the target microsphere indicated with an asterisk. Representative time-stamps in (A,B) were extracted from Videos S6 and S8 in Presentation 2 of Supplementary Material, respectively.