Figure 3.

Constitutively active- Phosph-Protein Kinase B (P-AKT) reduced Salmonella (S.C.)-induced decrease of MMP-9. The B16F10 and LL2 cells were transfected with constitutively active AKT plasmids for 16 h prior to 4 h Salmonella treatment. (a) The expression of phosphorylation AKT, AKT, phosphorylation Phosph-mammalian Targets Of Rapamycin (P-mTOR), mTOR, and MMP-9 protein in B16F10 and 4T1 cells was determined. The immunoblotting assay was repeated three times with similar results. The migration distances of B16F10 (b) and LL2 (c) cells were measured. (n = 3, data are mean ± SD. * p < 0.05).