Figure 4.

Salmonella (S.C.) reduced the function and expression of MMP-9 in vivo. The C57BL/6 mice had been subcutaneously inoculated with B16F10 and LL2 cells at day 0, and the tumor bearing mice were intraperitoneal injected with Salmonella (10⁶ cfu) at day 7. At day 10, the mice were sacrificed. The serum, tumors, livers, and spleen were collected. (a) The amounts of accumulated Salmonella in organs was determined; (b) The antitumor activity of Salmonella was determined by tumor weight. (n = 4, data are mean ± SD. * p < 0.05); The enzyme activity of MMP-9 in serum (c) and tumors (d) was determined by gelatin zymography; (e) The protein expression of MMP-9 in tumors derived from Salmonella-treated mice or control mice was determined by Western blotting.