Figure 2.

Inhibitory effect of IBC on mitotic clonal expansion. (A) Confluent 3T3-L1 preadipocytes were differentiated with MDI to induce differentiation in the presence or absence of IBC (40 μM). Cells were exposed to IBC during indicated differentiation periods. On D8, all groups were simultaneously subjected to ORO staining. ND, not-differentiated; MDI, differentiation medium. Scale bar = 100 μm; (B) Differentiation initiated adipocytes (D0) were treated with IBC for 48 h followed by flow cytometry; and (C) Cell lysates were prepared for Western blotting using indicated antibodies as described in Materials and Methods.