Table 2.
Putative AtSR1 target genes related to actions of growth hormones.
| Ratio | Direction | AGI Code | Functional Description | ChIP/SR1 | EMSA/SR1 |
|---|---|---|---|---|---|
| 2.04 | ↑ | At5g35735 | auxin-responsive family protein with unknown function | + | +,− |
| 1.57 | ↓ | At4g14560 | auxin-responsive protein/indoleacetic acid-induced protein 1 (IAA1) | + | −,+ |
| 0.44 | ↑ | At3g15540 | auxin-responsive protein/indoleacetic acid-induced protein 19 (IAA19) | + | + |
| 1.40 | ↑ | At1g16510 | SAUR41, small auxin up-regulated protein 41 | − | − |
| 1.27 | ↑ | At4g36110 | SAUR9, small auxin up-regulated protein 9 | + | + |
| 0.34 | ↓ | At3g61830 | ARF18, auxin-responsive factor gene family, AUX/IAA-related | + | +,+ |
| 1.34 | ↑ | At3g13380 | An LRR receptor-like kinase similar to BRI (BRL3) | + | + |
| 1.47 | ↓ | At1g75080 | brassinosteroid signaling positive regulator (BZR1) | + | - |
| 0.69 | ↓ | At3g50660 | steroid 22-alpha-hydroxylase (CYP90B1) (DWF4) | + | + |
“Ratio” = fold expression in sr1 as compared with WT; “Direction” = “↑” (increase) or “↓” (decrease) in expression; “ChIP/SR1” = chromatin immunoprecipitation (ChIP) assay of promoter of each gene interacting with full-length AtSR1 protein in planta; “EMSA/SR1” = electrophoretic mobility shift assay (EMSA) assay of each cg-box containing fragment interacting with cg1 domain of SR1. As a reference, the ratio of expression level (log2) of IAA1 (At4g14560) in atsr1 vs. WT is 1.57, direction is down-regulation.