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. 2018 Jun 12;19(6):1742. doi: 10.3390/ijms19061742

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Involvement of PIN1 pathway on effects of melatonin in EtOH-suppressed osteoblastic/cementoblastic differentiation in PDLCs and cementoblasts. Cells are pretreated with juglone (50 nM) or PIN1 siRNA (30 nM) and then incubated with melatonin (100 μM) and EtOH (25 mM) for 14 days (A–C). Differentiation was accessed by ALP activity (A), RT-PCR (B) and Alizarin red staining (C). These data are representative of three independent experiments. * statistically significant difference compared to the control groups (p < 0.05). ^# statistically significant difference in each group.