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Immunolocalization of JIM13 in the Brachypodium callus. (a–a”): control callus. (b–b”,c–c”): callus that has been treated with 5 µM of 5-azaC. (d–d”,e–e”): callus that has been treated with 50 µM of 5-azaC. The cells with a total absence of the signals are indicated by red arrows. Scale bars, 10 µm.
