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Histological sections of the Brachypodium callus stained with a 0.05% (w/v) toluidine blue O. (a–c): control callus; (d–f): callus that has been treated with 5 µM of 5-azaC. (g–i): callus that has been treated with 50 µM of 5-azaC. Scale bars, 10 µm.
