Cellular localization of 18kDa translocator protein (TSPO) expression in old vs. young mice after intracerebral hemorrhage (ICH). ICH was induced in male mice (20 months or 8 weeks old), as reported previously [27] and the brain sections were subjected to immunohistochemistry [27]. Briefly, brain sections were immunolabeled for (A) TSPO and Iba1 (microglia/macrophage marker); (B) TSPO and GFAP, (astrocyte marker) and (C) TSPO and NeuN (neuronal marker), 3 days-post ICH induction. A remarkable co-localization was observed between TSPO and Iba1, whereas no TSPO expression was observed in either NeuN or GFAP positive cells in both young as well as old mice. Scale Bar = 20 μM; n = 3 mice per group.