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. 2018 May 31;19(6):1633. doi: 10.3390/ijms19061633

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Further tuning the mechanical strength of DNA hydrogels by adding an ssDNA complementary to the aptamer sequence. (A) Characterization of C1B1 structure by 20% native PAGE. Four bands of ssDNAs B1 (Lane 1), B2 (Lane 2), R3 (Lane 3), and C1 (Lane 4) are clear. C1 can form structure with B1 with (Lane 6) or without B2 (Lane 8), while R1 cannot hybridize with B1 (Lane 7 and Lane 9); (B) Study of mechanical properties of the DNA hydrogel on adding ssDNA C1 and R1. All the measurements were carried out three times to get the average and statistical analysis was applied to get the error bar; and (C) Illustration of microscopic conformation transition of the aptamer sequence on adding ssDNA C1 and R1.