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. 2018 May 31;19(6):1626. doi: 10.3390/ijms19061626

Figure 1.

Figure 1

Hepatoprotective effect of oxaloacetate (OA) on human normal liver cells (LO-2 cells) against hydrogen peroxide (H2O2) injury. (A) The LO-2 cells were treated with 0.2, 0.4, 0.6, 0.8, 1, and 2 M H2O2 for 24 h. Cell viability was measured using an 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di- phenytetrazoliumromide (MTT) assay; (B) MTT assay. The LO-2 cells were treated with 5, 10, 20, 40, 80, and 100 mM OA for 24 h; (C) MTT assay. Groups were as described in “Methods: Cell line and Reagents”; (D) MTT assay. The LO-2 cells were pretreated with 10 mM different TCA substrates before treatment with 600 mM H2O2; (E) Cell microscopic morphology (200×) and colony formation. Groups were as described in “Methods: Cell line and Reagents”; (F) Annexin V-PI assay. Groups were as described in “Methods: Cell line and Reagents”. The column chart shows the percentage of apoptotic cells in each group. The experiments were repeated at least three times. The results were presented as the mean ± standard deviationc (SD). ** p < 0.01, *** p < 0.001 compared with the control group and ## p < 0.001 compared with H2O2-treated group.