Figure 2.

Study of proteasome activity in sensitive and resistance cell line. Measure of proteasome subunit 20S activity of the proteasome in MOLP8 (white) and MOLP8/R cell line (black histogram) (a) by using the kit Amplite Fluorimetric Proteasome 20S activity assay. Determination of the ubiquitination protein quantity in MOLP8 and MOLP8/R cell lines by Western blot (b). The left panel represents the PVDF membrane stained with the Ponceau Red, showing that the quantity of protein loading is the same for both cell lines. The right panel represents the photographic film resulting from the incubation of the membrane with ubiquitinated antibody overnight. ** p < 0.01.