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. 2018 May 24;19(6):1557. doi: 10.3390/ijms19061557

Figure 1.

Figure 1

hADSCs were seeded and myogenic differentiation was induced for 6 weeks. (a) Schematic diagram of the differentiation protocols used. Protocol C is the conventional or common protocol. Protocols M1–3 are modified as indicated. (b) mRNA expression of myosin heavy chain in hADSCs after 6 weeks of differentiation. n = 3 independent preparations, each assay performed in duplicate. All values are expressed relative to the gene expression observed using by protocol C (1 unit). All treatments are significantly different from each other (c) After 6 weeks of differentiation, cells were incubated with serum-free DMEM for 24 h, the media were collected, and IL-6 levels were measured. * p < 0.05 vs. protocol C. DMEM: Dulbecco’s Modified Eagle Medium; HS: horse serum; FBS: fetal bovine serum.