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Effect of a combination of IL-6 and C2C12 CM on morphological changes during differentiation and on IL-6 secretion. (a) Morphological changes of cells during the differentiation process. (b) After 4 weeks (protocol M3) or 6 weeks (protocol C) of differentiation, media were changed to serum-free DMEM and cells were incubated for 24 h. Media were collected and IL-6 levels were measured. w: week(s).
