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Effect of a colivelin on myogenic differentiation. (a–c) mRNA expression of (a) MyoD, (b) MyoG, and (c) MYH during myogenic differentiation. n = 3 independent experiments, each assay was performed in duplicate All values are expressed relative to the gene expression observed at 6 weeks using protocol C. * p < 0.05 vs. protocol C. (d) Cell morphology after 4 weeks of differentiation. (e) The expression of MYH and p-STAT3 was analyzed by western blotting.
