RGCs |
Anti-Brn-3a, 1:100 |
Santa Cruz Biotechnology, Heidelberg, Germany |
Alexa 488, 1:500 |
Dianova, Hamburg, Germany |
Apoptotic cells |
Anti-Bax, 1:100 |
Abcam, Cambridge, UK |
Alexa 555, 1:400 |
Invitrogen, Darmstadt, Germany |
Early autophagy |
Anti-LC3BII, 1:100 |
Cell Signaling, Danvers, MA, USA |
Alexa 488, 1:500 |
Invitrogen |
Late autophagy |
Anti-LAMP1, 1:100 |
Abcam |
Alexa 555, 1:400 |
Invitrogen |
Microglia |
Anti-Iba1, 1:400 |
Wako Chemicals, Neuss, Germany |
Alexa 488, 1:500 |
Invitrogen |
Activated microglia and macrophages |
Anti-ED1, 1:200 |
Millipore, Darmstadt, Germany |
Alexa 555, 1:500 |
Invitrogen |
Amacrine cells |
Anti-parvalbumin, 1:100 |
Swant, Marly, Switzerland |
Alexa 488, 1:500 |
Invitrogen |
Cholinergic amacrine cells |
Anti-ChAT, 1:250 |
Millipore |
Alexa 488, 1:500 |
Jackson Immuno Research, Cambridgeshire, UK |
Cone bipolar cells |
Anti-recoverin, 1:1000 |
Millipore |
Alexa 555, 1:400 |
Invitrogen |