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. 2018 May 31;19(6):1636. doi: 10.3390/ijms19061636

Table 1.

List of used primary and secondary antibodies, including cell type, dilution, and company.

Cell Type/Mechanism Primary Antibody with Dilution Company Secondary Anibody with Dilution Company
RGCs Anti-Brn-3a, 1:100 Santa Cruz Biotechnology, Heidelberg, Germany Alexa 488, 1:500 Dianova, Hamburg, Germany
Apoptotic cells Anti-Bax, 1:100 Abcam, Cambridge, UK Alexa 555, 1:400 Invitrogen, Darmstadt, Germany
Early autophagy Anti-LC3BII, 1:100 Cell Signaling, Danvers, MA, USA Alexa 488, 1:500 Invitrogen
Late autophagy Anti-LAMP1, 1:100 Abcam Alexa 555, 1:400 Invitrogen
Microglia Anti-Iba1, 1:400 Wako Chemicals, Neuss, Germany Alexa 488, 1:500 Invitrogen
Activated microglia and macrophages Anti-ED1, 1:200 Millipore, Darmstadt, Germany Alexa 555, 1:500 Invitrogen
Amacrine cells Anti-parvalbumin, 1:100 Swant, Marly, Switzerland Alexa 488, 1:500 Invitrogen
Cholinergic amacrine cells Anti-ChAT, 1:250 Millipore Alexa 488, 1:500 Jackson Immuno Research, Cambridgeshire, UK
Cone bipolar cells Anti-recoverin, 1:1000 Millipore Alexa 555, 1:400 Invitrogen