Table 2.
List of primer pairs used for analyses of RGCs, cell death mechanisms, and microglia mRNA expression in control, ischemic, untreated, as well as treated retinae by qRT-PCR. For relative quantification of mRNA levels, the house-keeping genes actin and cyclophilin were used. The primer sequence (5′–3′), the predicted amplicon size and the primer efficiency are indicated. Abbreviations: bp = base pairs, F = forward, R = reverse.
| Gene | Primer Sequence (5′–3′) | Amplicon Size | Primer Efficiency |
|---|---|---|---|
| β-Actin-F | cccgcgagtacaaccttct | 72 bp | 1.000 |
| β-Actin-R | cgtcatccatggcgaact | ||
| Cyclophilin-F | tgctggaccaaacacaaatg | 88 bp | 1.000 |
| Cyclophilin-R | cttcccaaagaccacatgct | ||
| Bad-F | caggcagccaataacagtca | 68 bp | 1.000 |
| Bad-R | tacgaactgtggcgactcc | ||
| Beclin-1-F | aggatggtgtctctcgaagatt | 77 bp | 0.969 |
| Beclin-1-R | gatcagagtgaagctattagcactttc | ||
| Brn-3a (Pou4f1)-F | ctggccaacctcaagatcc | 72 bp | 1.000 |
| Brn-3a (Pou4f1)-R | cgtgagcgactcgaacct | ||
| Caspase-3-F | ccgacttcctgtatgcttactcta | 70 bp | 1.000 |
| Caspase-3-R | catgacccgtcccttgaa | ||
| Caspase-9-F | cgtggtggtcatcctctctc | 81 bp | 1.000 |
| Caspase-9-R | gagcatccatctgtgccata | ||
| CD68-F | ctcacaaaaaggctgccact | 60 bp | 1.000 |
| CD68-R | ttccggtggttgtaggtgtc | ||
| ChAT-F | gcctcatctctggtgtgctt | 62 bp | 1.000 |
| ChAT-R | gtcagtgggaagggagtgg | ||
| Iba1-F | ctccgaggagacgttcagtt | 96 bp | 0.855 |
| Iba1-R | tttttctcctcatacatcagaatcatcagaat | ||
| LAMP1-F | ctgaaggtggggaacaagag | 66 bp | 1.000 |
| LAMP1-R | caggctagaagtggcattca | ||
| LC3B-F | tgaatatgagcgaactcatcaag | 92 bp | 1.000 |
| LC3B-R | catgctgtgcccattcac | ||
| NFκB-F | ctggcagctcttctcaaagc | 70 bp | 1.000 |
| NFκB-R | ccaggtcatagagaggctcaa | ||
| P62-F | ggggaccctgataacatcaa | 73 bp | 0.976 |
| P62-R | ccttgccggctaagatgag |