Figure 2.

Effects of 10⁻⁹ M melatonin with or without selective MT2 antagonists (10⁻⁹ M luzindole or 10⁻⁹ M 4-P-PDOT) on cumulus expansion at 44 h of IVM. The degree of cumulus expansion was classified into five groups, as described previously [30]. A total of 1344 cumulus-oocyte complexes was used in six independent replicates. Data are shown as the means ± SEM. Within each category, groups marked with different letters are significantly different (p < 0.05). Mtn, melatonin.