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Effects of 10−9 M melatonin with or without selective MT2 antagonists (10−9 M luzindole or 10−9 M 4-P-PDOT) on gene expression in porcine cumulus cells. (A) Cumulus expansion genes (Ptgs1, Ptgs2, Has2, Ptx3 and Tnfaip6); (B) Apoptosis gene (Bax/Bcl-2 ratio) and MT2. Data are shown as the means ± SEM. Within each category, groups marked with different letters are significantly different (p < 0.05). The experiment was replicated at least three times. Mtn, melatonin.
