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. 2018 Jun 14;19(6):1772. doi: 10.3390/ijms19061772

Figure 3.

Figure 3

The effects of phloretin on the phosphorylation of MAPKs in ST2 cells. (A–H) After reaching confluency, ST2 cells were incubated in adipogenic medium for 48 h. Thereafter, the cells were treated with 100 µM phloretin for up to 12 h, and western blot analysis was performed to examine the time-dependent effects of phloretin on the phosphorylation of ERK1/2, JNK, and p38 MAPK (A). To test dose dependency, the cells were treated with phloretin (0 to 100 µM) for 1 and 12 h (B). The results are representative of at least five experiments. (C–H) The quantification results are expressed as mean ± SE (n ≥ 5); * p < 0.05, ** p < 0.01, *** p < 0.001. Phl: phloretin.