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. 2018 Jun 14;19(6):1772. doi: 10.3390/ijms19061772

Figure 4.

Figure 4

The effects of the MEK inhibitor PD98059 on adipocyte differentiation in ST2 cells. (A,B) After reaching confluency, ST2 cells were incubated in adipogenic medium with the MEK inhibitor PD98059 (0, 10, and 20 µM) for 8 days. Thereafter, oil red O staining and its quantification were performed. The results are representative of at least seven different experiments. The quantification results are expressed as mean ± SE (n = 8); *** p < 0.001. (C–G) After reaching confluency, the cells were incubated in adipogenic medium with PD98059 (0, 10, and 20 µM) for 4 days. The mRNA expression of adipogenic differentiation markers (Pparγ, C/ebpα, Fas, Fabp4, and Apn) was examined by real-time PCR. The results are expressed as mean ± SE (n = 7); *** p < 0.001. PD: PD98059.