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. 2018 Jun 14;19(6):1772. doi: 10.3390/ijms19061772

Figure 6.

Figure 6

The effects of the p38 MAPK inhibitor SB203580 on phloretin-induced adipogenesis in ST2 cells. (A,B) After reaching confluency, ST2 cells were incubated in adipogenic medium with phloretin (50 µM) and/or SB203580, a p38 MAPK inhibitor (0, 5, and 10 µM) for 5 days. Thereafter, oil red O staining and its quantification were performed. The results are representative of at least seven different experiments. The quantification results are expressed as mean ± SE (n = 6); *** p < 0.001. (C–G) After reaching confluency, the cells were incubated in adipogenic medium with SB203580 (0, 5, and 10 µM) for 4 days. The mRNA expression of adipogenic differentiation markers (Pparγ, C/ebpα, Fas, Fabp4, and Apn) was examined by real-time PCR. The results are expressed as mean ± SE (n = 5); ** p < 0.01, *** p < 0.0011. Phl: phloretin, SB: SB203580.