Fig. 2.

Long-term hypoxia increases bradykinin-mediated cytosolic Ca²⁺ responses. Representative images (A) corresponding in time to the trace of fractional fluo 4 fluorescence (B) for four pulmonary arterial myocytes from a normoxic sheep in the presence of extracellular Ca²⁺. Lowercase letters denote the times at which the images occurred. The colored circles in A correspond to the trace of the same color in B. The brightness of the images in A was adjusted for display purposes. Images were made with a ×40 water immersion Apochromat objective at 1.28 frames/s. Scale bar is 20 μm.