Table 4.
Diacylglycerol molecular species levels in soleus muscle of normal diet/sedentary, normal diet/exercise-training, and high-fat diet/sedentary rats
|
m/z |
||||||
|---|---|---|---|---|---|---|
| Species | Precusor ion | Product ion | Adduct Ions | ND/Sed, nmol/g tissue | ND/Ex, nmol/g tissue | HFD/Sed, nmol/g tissue |
| 32:0 (16:0/16:0) | 586.54 | 313.27 | [M+NH4]+ | 3.70 ± 0.36 | 5.81 ± 0.53* | 3.68 ± 0.39 |
| 32:1 (16:1/16:0) | 584.53 | 313.27 | [M+NH4]+ | 3.11 ± 0.51 | 5.77 ± 0.87* | 2.09 ± 0.24 |
| 32:2 (16:1/16:1) | 582.51 | 311.26 | [M+NH4]+ | 0.344 ± 0.095 | 0.627 ± 0.191 | 0.115 ± 0.015* |
| 34:0 (16:0/18:0) | 614.57 | 341.31 | [M+NH4]+ | 1.25 ± 0.05 | 2.27 ± 0.19* | 2.27 ± 0.21* |
| 34:1 (16:0/18:1) | 612.56 | 339.29 | [M+NH4]+ | 30.7 ± 3.4 | 64.6 ± 6.5* | 41.8 ± 4.9 |
| 34:1 (16:1/18:0) | 612.56 | 341.31 | [M+NH4]+ | 0.417 ± 0.055 | 0.954 ± 0.142* | 0.504 ± 0.072 |
| 34:2 (16:0/18:2) | 610.54 | 337.27 | [M+NH4]+ | 14.9 ± 2.7 | 43.8 ± 5.6* | 46.4 ± 7.8* |
| 34:2 (16:1/18:1) | 610.54 | 339.29 | [M+NH4]+ | 7.3 ± 1.4 | 17.8 ± 3.0* | 7.2 ± 1.1 |
| 34:3 (16:1/18:2) | 608.53 | 337.27 | [M+NH4]+ | 0.80 ± 0.20 | 2.30 ± 0.38* | 1.36 ± 0.20 |
| 36:0 (18:0/18:0) | 642.60 | 341.31 | [M+NH4]+ | 0.284 ± 0.037 | 0.271 ± 0.018 | 0.301 ± 0.024 |
| 36:1 (18:1/18:0) | 640.59 | 341.31 | [M+NH4]+ | 3.69 ± 0.36 | 10.20 ± 1.11* | 11.38 ± 1.69* |
| 36:2 (18:1/18:1) | 638.57 | 339.29 | [M+NH4]+ | 28.5 ± 4.3 | 89.8 ± 11.4* | 65.0 ± 11.3* |
| 36:2 (18:2/18:0) | 638.57 | 341.31 | [M+NH4]+ | 8.2 ± 0.7 | 28.5 ± 3.6* | 33.2 ± 5.2* |
| 36:3 (18:2/18:1) | 636.56 | 339.29 | [M+NH4]+ | 21.7 ± 3.8 | 91.7 ± 12.9* | 98.8 ± 20.3* |
| 36:4 (16:0/20:4) | 634.54 | 361.27 | [M+NH4]+ | 3.40 ± 0.28 | 5.87 ± 0.50* | 5.14 ± 0.81 |
| 36:4 (18:2/18:2) | 634.54 | 337.27 | [M+NH4]+ | 1.37 ± 0.25 | 6.64 ± 0.94* | 9.42 ± 2.12* |
| 36:5 (16:1/20:4) | 632.53 | 361.27 | [M+NH4]+ | 0.130 ± 0.025 | 0.369 ± 0.061* | 0.198 ± 0.046 |
| 38:4 (18:0/20:4) | 662.57 | 361.27 | [M+NH4]+ | 31.7 ± 1.9 | 40.3 ± 2.5* | 36.4 ± 1.7 |
| 38:5 (18:1/20:4) | 660.56 | 361.27 | [M+NH4]+ | 3.20 ± 0.43 | 8.33 ± 0.99* | 8.02 ± 1.65* |
| 38:6 (16:0/22:6) | 658.54 | 385.27 | [M+NH4]+ | 2.01 ± 0.38 | 5.05 ± 0.54* | 4.40 ± 0.92* |
| 38:6 (18:2/20:4) | 658.54 | 361.27 | [M+NH4]+ | 0.52 ± 0.11 | 2.12 ± 0.34* | 3.86 ± 0.95* |
| 38:7 (16:1/22:6) | 656.53 | 385.27 | [M+NH4]+ | 0.246 ± 0.066 | 0.779 ± 0.123* | 0.477 ± 0.110 |
| 40:6 (18:0/22:6) | 686.57 | 385.27 | [M+NH4]+ | 2.40 ± 0.31 | 5.90 ± 0.59* | 5.07 ± 0.79* |
| 40:7 (18:1/22:6) | 684.56 | 385.27 | [M+NH4]+ | 1.88 ± 0.53 | 7.24 ± 1.01* | 6.32 ± 1.52* |
| 40:8 (18:2/22:6) | 682.54 | 385.27 | [M+NH4]+ | 0.54 ± 0.15 | 2.61 ± 0.40* | 3.17 ± 0.78* |
| 40:8 (20:4/20:4) | 682.54 | 361.27 | [M+NH4]+ | 0.221 ± 0.042 | 0.650 ± 0.107* | 0.993 ± 0.246* |
Data are presented as means ± SE. ND/Sed, normal diet/sedentary rats; ND/Ex, normal diet/exercise-training rats; HFD/Sed, high-fat diet/sedentary rats.
*
P < 0.05 compared with the ND/Sed rats by Dunnett's multiple comparison test.