Table 5.
Expression of BrHSP18.2 family genes in Chiifu and Kenshin under various temperature conditions. Expression level (fold change) was calculated based on qRT-PCR values of three replicates using BrACT2 as a standard. Heat shock treatment was performed by increasing the temperature 5 °C every 2 h.
| Gene | Line | Expression (Fold Change) | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Heat Shock | Warming | |||||||||
| 22 °C | 27 °C | 32 °C | 37 °C | 42 °C | 22 °C | 12 °C | 28 °C | 45 °C | ||
| BrHSP18.2A | Chiifu | 1.0 | 2.2 | 10.3 | 3220.4 | 4132.9 | 1.0 | 0.5 | 8.4 | 7154.1 |
| Kenshin | 1.0 | 0.8 | 5.5 | 4921.5 | 49,617.7 | 1.0 | 0.5 | 106.7 | 34,142.0 | |
| BrHSP18.2B | Chiifu | 1.0 | 0.5 | 8.7 | 74.4 | 357.9 | 1.0 | 1.2 | 1.3 | 241.4 |
| Kenshin | 1.0 | 3.9 | 5.7 | 160.5 | 1738.6 | 1.0 | 1.8 | 42.3 | 31,296.8 | |
| BrHSP18.2C | Chiifu | 1.0 | 1.4 | 13.8 | 140.0 | 646.8 | 1.0 | 1.8 | 7.8 | 7540.8 |
| Kenshin | 1.0 | 2.3 | 4.0 | 84.1 | 2960.7 | 1.0 | 1.5 | 3.1 | 733.5 | |