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. 2018 May 24;19(6):1567. doi: 10.3390/ijms19061567

Figure 2.

Figure 2

Figure 2

Luteolin induces hepatic stellate cell (HSC) apoptosis and attenuates α-smooth muscle actin (α-SMA) expression. (a) Fluorescence staining for Yo-Pro-1 and PI after treatment with vehicle or 40 µM of luteolin for 24 h in both LX-2 and HSC-T6 cells; (b) Immunofluorescence staining for α-SMA after treatment with vehicle or 40 µM of luteolin for 24 h in LX-2 cells; (c) Western blot with LX-2 whole cell lysate after incubation for 24 or 48 h with vehicle or varying concentrations of luteolin for α-SMA. The results are representative of at least 3 independent experiments.