Figure 1.

IL-10-producing B cells after B-cell stimulation. B cells were treated for 48 h and analysed by flow cytometry. (A) Total IL-10-producing cells were analysed within viable cells using intracellular labelling in non-treated (NT) and CpG-treated B cells (dot plots). Dot plot of one representative experiment out of five is shown. Numbers represent the percentages of living cells and total IL-10-producing B cells. (B) Average percentages of IL-10-producing viable non-stimulated or stimulated B cells was analysed. Cultures were performed in the presence of IL-1β, CD40L, GM-CSF, or CpG. Average + SEM of five experiments for each condition are shown. * p < 0.05 when comparing non-treated condition versus treated condition.