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. 2018 May 24;19(6):1562. doi: 10.3390/ijms19061562

Figure 3.

Figure 3

Surface plasmon resonance spectra showing direct interaction between the Cx43CT and β-catenin CT domains. Cx43CT was immobilized onto a CM5 chip by amine coupling (Biacore; GE Healthcare, Uppsala, Sweden) and either β-catenin CT (500 response units), Cx43EL1 (negative control), or Cx43CT (residues S255–I382, positive control) were flown over the chip as indicated on the top of the graph. The chip was regenerated after an interaction was observed. Repeat of an injection of β-catenin CT was performed to confirm the interaction.