Figure 3.

MK-0677-treated 5XFAD mice exhibited a significant reduction in neurodegeneration compared with the vehicle group. Immunofluorescent staining was performed to detect the markers of neuronal cells (NeuN) and pre-synaptic terminals (SYN) in layer V of the frontal cortex of wild-type (n = 8) and 5XFAD mice. MK-0677 significantly ameliorated the reduction of the number of NeuN (+) cells (A) and optical density of SYN (+) area (B) in 5XFAD mice (n = 6), compared with vehicle-treated 5XFAD mice (n = 7). *** p < 0.001 indicates significant differences between the groups. Scale bars are 50 μm in the upper panel and 25 μm in the lower panel.