Figure 13.

Nanomolar (nM) concentrations of Cur inhibit Aβ42 aggregation in vitro. HFIP-treated Aβ42 was incubated with and without different concentrations of Cur for 24–72 h and a dot blot was performed by probing with Aβ42-fibril specific antibody (OC) and the color was developed with chemiluminescent reagents and the optical density of each dot was measured using Image-J software. Lower concentrations (1–0.001 µM) of Cur inhibited Aβ42 aggregation, whereas higher concentrations had no effect on aggregation.