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Effects of vaspin on ECM expression in HASMCs were assessed by immunoblotting. The blottted membranes were cut for reacting with different antibodies, and the blots were stripped and reprobed in the same membrane. Top: Representative results of protein expression of collagen 1 (A), collagen 3 (B), fibronectin (C), elastin (D), and MMP2 (E). Bottom: Densitometric data of each molecule after normalization relative to α-tubulin (n = 3–4). * p < 0.05 vs. 0 ng/mL of vaspin.
