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. 2018 May 24;19(6):1558. doi: 10.3390/ijms19061558

Figure 3.

Figure 3

Silencing Pannexin-1 expression affects capillary-like formation by HDLECs. (A) Representative immunoblots of HDLECs extracts prepared 48 h after transfection with either the control or the PANX1-specific siRNAs. GAPDH blot served as the loading control; Bar graph shows the quantification of PANX1 expression loss 48 h after siRNA transfection. Data represent the mean ± SD from four independent experiments; (B) Representative immunoblots and densitometric quantification of PANX1, PANX2 and PANX3 expression from HDLECs extracts prepared 48 h after transfection with either the control or the PANX1-specific siRNAs. GAPDH blot served as the loading control; (C) Representative images of tube structure formation in HDLECs on Matrigel after transfection. Cells transfected with PANX1 siRNAs showed defects in capillary network formation; (D) Quantitative analysis for total length of tubule complexes and total number of junctions per field in control and PANX1 siRNA-transfected HDLECs. Data represent the mean ± SD from six independent experiments conducted in duplicate. * p < 0.05, ** p < 0.01 and *** p < 0.001.