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. 2018 Jun 5;19(6):1678. doi: 10.3390/ijms19061678

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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NPC2 expression and free cholesterol accumulation were not involved in the regulation of HSC apoptosis. (A) Protein expression of cleaved caspase 9, full caspase 9, cleaved caspase 3, full caspase 3, cleaved PARP, full PARP, LC3B, and α-tubulin was analyzed by using immunoblotting in NPC2-downregulated HSC-T6, NPC2-overexpressed LX2 and U18666A-treated LX2 cells; (B) Gene expression of pro-apoptotic Bax and anti-apoptotic Bcl-xl was analyzed by using real-time PCR in NPC2-downregulated HSC-T6 cells, NPC2-overexpressed LX2 cells, and U18666A-treated LX2 cells. Data are shown as mean ± SD. Each experiment was performed using three independent replicates. A similar phenomenon was observed, and representative data are shown in the figure.