Figure 4.

Using dynamic force spectroscopy to investigate αSyn_71–82 dissociation. (A) Schematic representation of an unbinding energy landscape. The dotted line depicts the tilted energy landscape under force. x _U is depicted as the distance from the bound energy well to the transition state. $k_{\mathrm{o}\mathrm{f}\mathrm{f}}^{0F}$ is shown as the stochastic process of crossing the transition energy barrier. (B) Dynamic force spectrum of αSyn_71–82 dissociation. Data (mean of the triplicate datasets, error bars are the standard deviation) are fitted to the Bell‐Evans model45, 46 to obtain values $k_{\mathrm{o}\mathrm{f}\mathrm{f}}^{0F}$ and x _U (shown inset). The errors for $k_{\mathrm{o}\mathrm{f}\mathrm{f}}^{0F}$ and x _U were calculated by manual bootstrapping. The total number of approach retract cycles (total triplicate) for pL αSyn_71–82 dimer dissociation events are 5200, 5500, 6500, 6000, and 5500 for pulling speeds 200, 500, 1000, 3000, and 5000 nms⁻¹, respectively.