Figure 1.

Efficient ligation of cyclic signal transducer and activator of transcription 3 (STAT3) decoy (CS3D). (A) Schematic representation of CS3D ligation with T4 DNA ligase. The complementary segments of the single-stranded decoy molecule spontaneously self-anneal. Enzymatic ligation with T4 DNA ligase was used to complete cyclization. (B) Incubations were performed in the absence or presence of T4 DNA ligase overnight. Multiple identical ligations (n = 5) were simultaneously performed. Samples from each reaction were then electrophoresed on a urea/polyacrylamide gel, stained with SYBR Gold, and quantified by densitometry.