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. 2018 May 30;19(6):1608. doi: 10.3390/ijms19061608

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Diagram of the serum stability assay. (1) Incubate biotinylated oligodeoxynucleotide (ODN) in fresh human serum. (2) Remove aliquots of ODN/serum mixture at various time points and store in −80 °C. (3) Incubate streptavidin resin with thawed aliquots of ODN/serum mixture. (4) Pellet and wash three times. (5) Elute by boiling, separate on urea/polyacrylamide gels, and develop with ultraviolet light.